COURSE OVERVIEW
Who is this course for?
This course is designed for those relatively new to GC and ideally will follow-on from attendance to the Fundamental GC training course.
Previous Knowledge
Some previous knowledge of gas chromatography and basic experience are recommended but not essential.
What you will learn
- How the chromatographic process takes place
- The architecture of a GC system
- Working principles and problems associated with each component of the system
- How to use chromatograms to troubleshoot chromatographic problems
- Best practices for column use and care
- Best practice in system maintenance and performance evaluation
COURSE OUTLINE
Approaches to Logical Troubleshooting
- Systematic Troubleshooting Strategies
- Understanding the GC System as a Whole
- Component-Specific Troubleshooting
- Using System Maintenance Records for Diagnosis
- Identifying Symptoms, Causes, and Solutions
Component-Specific Troubleshooting
- Injectors: on-column, split – splitless, large volume injection
- Detectors: FID, ECD, NPD, FPD
- Temperature and pressure control Failures
Column Maintenance and Optimization
- Proper Installation and Conditioning of Columns
- Operating principles and Best Practices
- Bleed Prevention and Managing Column Degradation
- Operating range/bleed
- Preventing Band Broadening and Peak Distortion
Symptomatic Troubleshooting: Baseline Issues
- Identifying and Resolving Baseline Spikes
- Troubleshooting Noisy Baselines
- Addressing Cycling and Drifting Baselines
Symptomatic Troubleshooting: Peak Issues
- No Peaks or Poor Peak Shape
- Fronting, Tailing, and Split Peaks
- Identifying and Correcting Broad Shoulders and Ghost Peaks
- Ensuring Retention Time Stability
- Addressing Solvent Incompatibility Issues
- Loss of Sensitivity and Peak Response
Preventive Maintenance for GC Systems
- Establishing Routine Maintenance Schedules
- Proper Injector and Detector Maintenance Procedures
- Best Practices for Long-Term System Performance
Q&A and Closing Discussion
- • Summary of Key Concepts
- • Open Discussion on Challenges Faced in Derivatization
- • Resources for Further Learning